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--- nikon_eclipse_lv150_optical_microscope [2023/03/29 14:29] – scholma
+++ nikon_eclipse_lv150_optical_microscope [2024/12/18 12:30] (current) – wigbout
@@ Line 1: / Line 1: @@
 ==== Nikon ECLIPSE LV150 optical microscope ====
+Manufacturers Manual: {{ :nikon_lv150-150a_manual.pdf |Nikon ECLIPSE LV150 Optical Microscope}}.
-The optical microscope in the cleanroom is a Nikon ECLIPSE LV150 optical microscope.
+=== Lamp ===
+The microscope lamp is a (LV-HL50W) 12V50W halogen lamp with uniform light intensity distribution across the filament area. Filament-based lamps have a finite life-time, so **make sure to turn off the light** when you are done with the optical microscope.
-The manufacturers manual can be found here: {{ :nikon_lv150-150a_manual.pdf |}}
+=== Stage ===
+Since acetone can dissolve many plastics, **do not spill any acetone on the stage**. If you want to observe a lift-off process, you could use put a small petri dish in a larger petri dish, so that if the acetone spills from the smaller petri dish, it will not land on the stage.
-=== DO's and DONT's===
+It sometimes occurs that the X/Y-stage is stuck. If it happens, and the linear actuators are 'jittery' (i.e., the actuator moves back and forth without a sum translation occurring), it helps to restart the controller.
-  * DO NOT change the calibration settings!
-  * DO NOT spill Aceton on the microscope and microscope stage, Aceton can dissolve many plastics
+If the stage moves freely, the nut came loose from the spindle. This can happen when the nut is not tightened enough. Check if this is the case by removing the black cover panels. Try to tighten the nut on the spindle.
-  * DO turn off the light when leaving the microscope, the lamp has a finite lifetime
+(In the case that the Y-actuator is not working, it is easier to remove the stage from the mount. The nut cannot be seen from the top, so by removing the stage from the microscope, it is a bit easier to turn the stage upside down and inspect the nut-spindle situation. When removing the stage, be //extremely careful// as not to hit the objective lenses with the stage and potentially damaging them. It would be best to remove the nose of objective lenses first. How this is done, can be found in the manual linked above.)
-  *
+Sometimes, when the actuators are not operating properly, or failing every two or three days, the actuators just need a bit of grease.
 === Saving an image with annotations ===
-To save annotations/measurements on an image, first capture the image. Make sure the correct objective is selected at which you took the picture, otherwise the measurements will be off.
+To save annotations/measurements on an image, first capture the image. Make sure the correct objective is selected at which you took the picture, otherwise the measurements will be off.\\
-Use one of the drawing tools on the right side of the GUI. Use the option 'Burn overlay', shortcut key 'Y'. Now the annotations are saved in the image and you can save the image.
+Use one of the Annotations and measurement tools to make your measurements. Use the option 'Burn overlay', shortcut key 'Y', to burn the annotation layer into your captured image. Now you can save the image.
 === Stitching images together, creating a sample overview ===