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--- thermofisher_apreosem [2025/05/02 11:25] – wigbout
+++ thermofisher_apreosem [2025/05/02 11:27] (current) – [No image?] wigbout
@@ Line 1: / Line 1: @@
 {{ :apreosem_manual.pdf |}}
+==== Instructions.txt ====
+- Use gloves and mount sample on a semstub (either carbon- or coppertape, or carbonpaint)
+- Big sized objects (in vertical direction!!) or heavy objects should be discussed with a technician (see contacts below!).
+- Check the CCD camera and look if something is off (other samples in or stage tilted etc.), check previous logbook entry for any comments.
+- Write in logbook.
+- If you have a non-standard sized object in the Z direction lower the stage (don't forget the ESC-button in case of emergency).
+- Vent.
+- Set the working folder (D:\User\<yourname>) on the Support-PC
+- Once the green bar at the bottom is at 50% you should be able to open the door.
+- Look at the camera if you open/close the door!
+- Mount sample (wearing gloves, of course).
+- Push the door a bit to make sure its closed.
+- If you have a MAGNETIC MATERIAL then choose the option magnetic sample in the sample exchange window!
+- Make sure to enable 'Take Nav-Cam Photo' (otherwise, do it manually once the chamber is pumped: Stage > Take Nav-Cam Photo)
+- Pump (and choose no accessory).
+- Change working folder (\\AO106-SPC\User\[yourname]) (also called the (Z:))
+ (in case it doesnt work; change it to D:/User and after you are done, transfer data using this computer (shortcut on desktop)
+- Change use case to standard.
+- Change detector to the ETD.
+- Choose SEM settings HV and beam current (default: 15 kV, 0.10nA, insulators: 2kV, 13pA)
+- Check if the pressure in chamber pressure <9.9e-5
+- Turn on the beam
+- Go to the highest sample and get a clear image. (Image black/white? Check the contrast/brightness (F3). Still black? Check detector.)
+- Have a good focus at >20000x magnification. (This makes sure that the error in your calculated WD is small.)
+- Press 'Link Z to FWD' (top toolbar, icon with red questionmark)
+- Move the stage to 10 mm (while holding your finger above the ESC button to stop the stage)
+- Lock the Z-axis.
+- Either change the use case now or start imaging in the standard mode.
+- Gather data (use either data03 or surfdrive to transfer)
+- Beam off
+- Fill in logbook
+- Set parameters back to normal (standard mode/etd)
+- Vent (once halfway again you can open the chamber while watching the screen again)
+- Pump down (no accessory)
+- Check that the chamber pressure goes down (if not wait for it and give a tiny push to the door)
+- Wait until pressure is ~1e-4 mbar
+- Copy your files from the working folder (D:\User\<yourname>) to microscope (\\data03.physics.leidenuniv.nl\microscope\<yourname>) if necessary.
+- Clean up your mess
+in case something non-standard happens contact Luc Wigbout tel: 071 527 5448, mail: wigbout@physics.leidenuniv.nl
+For LION members, copy data to data03.